Mortality of Diatraea saccharalis is affected by the pH values of the spore suspension of Beauveria bassiana and Metarhizium anisopliae

Fungal virulence is multifaceted and dependent on multiple factors including the pH of spore suspension. In this study, we accessed effects six values Beauveria bassiana, Metarhizium anisopliae medium for growth, sporulation, mortality sugarcane stalk borer Diatraea saccharalis. The culture fungi was performed onplates containing PDA (Potato Dextrose Agar) medium. Virulence tested in D. saccharalis larvae distributed four replicates 15 larvae. To evaluate performance isolates, they were grown at different an artificial chitin to confirm degradation capacity each pH. No significant difference observed sporulation ranged from 4 9 both fungi. assay, larval higher 7 8 fungi, reaching 87% B. bassiana 81% M. anisopliae.